Our results suggest that CAP59 is involved in capsule synthesis by participating in the process of GXM (polysaccharide) export. Furthermore, the cell diameter and volume for strain C536 are higher than those for strain B-3501 or B-4131 and may suggest the accumulation of cellular material in the cytoplasm. In its yeast state, it is often found in bird excrement. Its teleomorph is a filamentous fungus, formerly referred to Filobasidiella neoformans. Cryptococcus neoformans, an encapsulated organism, is a narrow-based budding yeast, as seen on histological staining with India ink, haematoxylin and eosin, Grocott-Gomoris methenamine silver, or. Hence, the missense mutation of CAP59 in B-4131 partially hampered the trafficking of GXM but allowed the secretion of enzymes involved in hydrolysis of raffinose or urea. Cryptococcus neoformans is an encapsulated yeast 1 belonging to the class Tremellomycetes and an obligate aerobe 2 that can live in both plants and animals. In addition, the cells of B-3501 and B-4131, but not those of the cap59 deletant, assimilated raffinose or urea. Note the clear zone of the capsule around the. Furthermore, the epitopes recognized by MAbs 12A1, 2D10, 13F1, and 18B7 were each detected in the cytoplasm of all strains by immunogold electron microscopy, although there were differences in location consistent with differences in epitope synthesis and/or transport. This is an India ink preparation of cerebrospinal fluid in a patient with Cryptococcus neoformans meningitis. Analysis of cell lysates for GXM by enzyme-linked immunosorbent assay revealed GXM in C536 samples. Other stains: Mucicarmine stain which stains carminophilic cell wall of C. Microscopic examination: India ink preparation.
#INDIA INK CRYPTOCOCCUS NEOFORMANS SKIN#
Specimen: It depends on the site of infection and the common sample are CSF, sputum, blood, pus, brain tissue, and skin scrapings. The cap59 gene deletion mutant (C536), however, did not show a capsule by either India ink staining or immunofluorescence. Laboratory Diagnosis of Cryptococcus neoformans. B-4131, a mutant containing a missense mutation in CAP59, showed no capsule by India ink staining but revealed the presence of capsular polysaccharide on the cell surface by immunofluorescence. Wild-type strain B-3501 showed a visible capsule by India ink staining and immunofluorescence with anticapsular monoclonal antibodies (MAbs) 12A1 and 18B7. We examined the localization of glucuronoxylomannan (GXM) in strain B-3501 and in cap59 mutants B-4131 and C536. Several genes are essential for Cryptococcus neoformans capsule synthesis, but their functions are unknown.
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